Stimulated emission depletion microscopy with array detection and photon reassignment
نویسندگان
چکیده
منابع مشابه
Stimulated emission depletion microscopy with optical fibers
Imaging at the nanoscale and/or at remote locations holds great promise for studies in fields as disparate as the life sciences and materials sciences. One such microscopy technique, stimulated emission depletion (STED) microscopy, is one of several fluorescence based imaging techniques that offers resolution beyond the diffractionlimit. All current implementations of STED microscopy, however, ...
متن کاملSupraresolution Imaging in Brain Slices using Stimulated-Emission Depletion Two-Photon Laser Scanning Microscopy
Two-photon laser scanning microscopy (2PLSM) has allowed unprecedented fluorescence imaging of neuronal structure and function within neural tissue. However, the resolution of this approach is poor compared to that of conventional confocal microscopy. Here, we demonstrate supraresolution 2PLSM within brain slices. Imaging beyond the diffraction limit is accomplished by using near-infrared (NIR)...
متن کاملTime-resolved stimulated emission depletion in two-photon excited states.
A new approach to time-resolved fluorescence spectroscopy based on stimulated emission depletion (STED) of two-photon excited states is presented. Combined with time-resolved detection, STED can circumvent orientational averaging constraints and spontaneous emission selection rules inherent in all conventional techniques. Applications of STED to the study of molecular probe dynamics are present...
متن کاملSpectroscopic rationale for efficient stimulated-emission depletion microscopy fluorophores.
We report a rationale for identifying superior dyes for stimulated-emission depletion (STED) microscopy. We compared the dyes pPDI and pTDI, which displayed excellent photostability in single-molecule spectroscopy. Surprisingly, their photostability and performance in STED microscopy differed significantly. While single pTDI molecules could be visualized with excellent resolution (35 nm), pPDI ...
متن کاملStimulated Emission Depletion (STED) Microscopy: from Theory to Practice
In recent years, various methods have been proposed to increase the resolution of fluorescence microscopy beyond the diffraction limit of light. Amongst them, Stimulated Emission Depletion (STED) microscopy, which relies on a purely physical concept of fluorescence depletion could, in theory, achieve unlimited resolution independent of the wavelength of light. In this chapter, we review the fun...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Optics and Lasers in Engineering
سال: 2020
ISSN: 0143-8166
DOI: 10.1016/j.optlaseng.2020.106061